Animal-Free Cytokines

Animal-Free Recombinant Human GDNF

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Product Details

Catalogue Number: AF-450-10
Description:

GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin.  These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures.  GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors.  GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons.  Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability.  The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers.  Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Glial-Derived Neurotrophic Factor, ATF-1

AA Sequence (monomer): MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 30.4 kDa

Accession Number: P39905

Gene ID: 2668

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

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Crutcher, E
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mTOR and autophagy pathways are dysregulated in murine and human models of Schaaf-Yang syndrome.
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Scientific Reports; 9(1) pg15935
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Intrinsic Immunity Shapes Viral Resistance of Stem Cells.
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Cell; 172(3) pg423-438.e25
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Rousseaux, M W
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ATXN1-CIC Complex Is the Primary Driver of Cerebellar Pathology in Spinocerebellar Ataxia Type 1 through a Gain-of-Function Mechanism.
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Neuron; 97(6) pg1235-1243.e5
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