Animal-Free Recombinant Human GDNF 0 ReviewsSubmit a Review Product Details Catalogue Number: AF-450-10 Description: GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Glial-Derived Neurotrophic Factor, ATF-1 AA Sequence (monomer): MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 30.4 kDa Accession Number: P39905 Gene ID: 2668 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) crossreactivity: Human, Monkey, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Cell Culture Immune System Neurobiology Stem Cells & Differentiation TGF-β Superfamily product.subtitle.recentcitations First Author Özcan, A Title Programmable RNA targeting with the single-protein CRISPR effector Cas7-11 Citation Nature; 597(7878) pg720-725 PubMed Id 34489594 First Author Brighi, C Title Novel fragile X syndrome 2D and 3D brain models based on human isogenic FMRP-KO iPSCs. Citation Cell death & disease; 12(5) pg498 PubMed Id 33993189 First Author Ke, M Title Azoramide protects iPSC-derived dopaminergic neurons with PLA2G6 D331Y mutation through restoring ER function and CREB signaling Citation Cell Death & Disease; 11(2) pg130 PubMed Id 32071291
