Animal-Free Recombinant Human GM-CSF 1 ReviewsSubmit a Review Product Details Catalogue Number: AF-300-03 Description: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant Human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Granulocyte-Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α AA Sequence: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 14.6 kDa Accession Number: P04141 Gene ID: 1437 crossreactivity: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 Cancer Cell Culture Immune System Inflammation Stem Cells & Differentiation Wound Healing product.subtitle.recentcitations First Author Spadaro, F Title IFN-α enhances cross-presentation in human dendritic cells by modulating antigen survival, endocytic routing, and processing. Citation Blood; 119(6) pg1407-17 PubMed Id 22184405 First Author Hosszu, K K Title DC-SIGN, C1q, and gC1qR form a trimolecular receptor complex on the surface of monocyte-derived immature dendritic cells. Citation Blood; 120(6) pg1228-36 PubMed Id 22700724 First Author Pavel-Dinu, M Title Gene correction for SCID-X1 in long-term hematopoietic stem cells. Citation Nature Communications; 10(1) pg1634 PubMed Id 30967552 product.subtitle.productreviews Existing reviews Very good experience We use GM-CSF to differentiate human and rabbit macrophages and we can highly recommend it. From: Hanna-Mari | Date: 19/08/2020 × Very good experience We use GM-CSF to differentiate human and rabbit macrophages and we can highly recommend it. From: Hanna-Mari | Date: 19/08/2020 Close