Animal-Free Cytokines

Animal-Free Recombinant Human GM-CSF

Product Details

Catalogue Number: AF-300-03
Description:

GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant Human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Granulocyte-Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α

AA Sequence: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 14.6 kDa

Accession Number: P04141

Gene ID: 1437

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Spadaro, F
Title
IFN-α enhances cross-presentation in human dendritic cells by modulating antigen survival, endocytic routing, and processing.
Citation
Blood; 119(6) pg1407-17
PubMed Id
First Author
Hosszu, K K
Title
DC-SIGN, C1q, and gC1qR form a trimolecular receptor complex on the surface of monocyte-derived immature dendritic cells.
Citation
Blood; 120(6) pg1228-36
PubMed Id
First Author
Pavel-Dinu, M
Title
Gene correction for SCID-X1 in long-term hematopoietic stem cells.
Citation
Nature Communications; 10(1) pg1634
PubMed Id

product.subtitle.productreviews

Existing reviews
Very good experience
We use GM-CSF to differentiate human and rabbit macrophages and we can highly recommend it.
From:  Hanna-Mari | Date:  19/08/2020