Animal-Free Cytokines

Animal-Free Recombinant Human IGF-I

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Product Details

Catalogue Number: AF-100-11
Description:

The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro.  IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times.  The IGFs belong to the Insulin gene family, which also contains insulin and relaxin.  The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin.  IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone.  Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor.  Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.  Recombinant Human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. The calculated molecular weight of Recombinant Human IGF-I is 7.6 kDa.     

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Insulin-like Growth Factor-I, Somatamedin C, IGF-IA

AA Sequence: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 7.6 kDa

Accession Number: P05019

Gene ID: 3479

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
James, O G
Title
iPSC-derived myelinoids to study myelin biology of humans.
Citation
Developmental Cell; 56(9) pg1346-1358.e6
PubMed Id
First Author
Medyouf, H
Title
High-level IGF1R expression is required for leukemia-initiating cell activity in T-ALL and is supported by Notch signaling.
Citation
The Journal of Experimental Medicine; 208(9) pg1809-22
PubMed Id
First Author
Wilson, T R
Title
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Citation
Nature; 487(7408) pg505-9
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