Animal-Free Cytokines

Animal-Free Recombinant Human IGF-II

0 ReviewsSubmit a Review

Product Details

Catalogue Number: AF-100-12
Animal-Free Recombinant Human IGF-II

The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro.  IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times.  The IGFs belong to the Insulin gene family, which also contains insulin and relaxin.  The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin.  IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone.  Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor.  Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.  Recombinant Human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds.  The calculated molecular weight of Recombinant Human IGF-II is 7.5 kDa.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Insulin-like Growth Factor-II, Somatamedin A


Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to stimulate the proliferation of mouse FDC-P1 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Animal-Free Recombinant Human IGF-II Biological Activity Graph

Calculated Molecular Weight: 7.5 kDa

Accession Number: P01344

Gene ID: 3481

Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug)

Country Of Origin: USA

Not for human use.

Research Interest