Animal-Free Cytokines

Animal-Free Recombinant Human IL-1β

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Product Details

Catalogue Number: AF-200-01B
Description:

IL-1β is a proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. Both IL-1α and IL-1β bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine.  Recombinant Human IL-1β is a 17.3 kDa protein containing 153 amino acid residues.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)

AA Sequence: APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to stimulate the proliferation of mouse D10S cells. The expected ED50 is ≤ 0.001 ng/ml, corresponding to a specific activity of ≥ 1 x 109 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 17.3 kDa

Accession Number: P01584

Gene ID: 3553

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Murahashi, Y
Title
Intra-articular administration of IκBα kinase inhibitor suppresses mouse knee osteoarthritis via downregulation of the NF-κB/HIF-2α axis.
Citation
Scientific Reports; 8(1) pg16475
PubMed Id
First Author
Rabenhorst, A
Title
Mast cells play a protumorigenic role in primary cutaneous lymphoma.
Citation
Blood; 120(10) pg2042-54
PubMed Id
First Author
Rossato, M
Title
IL-10-induced microRNA-187 negatively regulates TNF-α, IL-6, and IL-12p40 production in TLR4-stimulated monocytes.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 109(45) pgE3101-10
PubMed Id