Animal-Free Cytokines

Animal-Free Recombinant Human IL-10

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Product Details

Catalogue Number: AF-200-10
Description:

IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes.  IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-α.  Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions.  Human IL-10 is active on murine cells, but murine IL-10 is inactive on human cells.  Recombinant Human IL-10 is an 18.6 kDa protein of 161 amino acid residues.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: B-TCGF, CSIF, TGIF

AA Sequence: MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by the dose-dependent co-stimulation (with human IL-4) of MC/9 cells.  The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 18.6 kDa

Accession Number: P22301

Gene ID: 3586

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Yamashiro, C
Title
Benzalkonium chloride-induced myofibroblastic transdifferentiation of Tenon's capsule fibroblasts is inhibited by coculture with corneal epithelial cells or by interleukin-10
Citation
Scientific Reports; 11(1) pg16096
PubMed Id
First Author
Braun, D A
Title
Cytokine response is determined by duration of receptor and signal transducers and activators of transcription 3 (STAT3) activation.
Citation
The Journal of Biological Chemistry; 288(5) pg2986-93
PubMed Id
First Author
Zhao, M
Title
IL-10 reduces levels of apoptosis in Toxoplasma gondii-infected trophoblasts.
Citation
PLoS ONE; 8(2) pge56455
PubMed Id