Recombinant Human IL-4 3 ReviewsSubmit a Review Product Details Catalog Number: 200-04 Description: IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant Human IL-4 is a 15.1 kDa globular protein containing 130 amino acid residues. Source: E.coli Synonyms: BCGF, BCDF, B cell stimulating factor (BSF-1) AA Sequence: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 as determined by the dose-dependent stimulation of human TF-1 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 15.1 kDa Accession Number: P05112 Gene ID: 3565 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Cross Reactivity Cited in References: Bacteria, Chicken, Human, Human + Mouse, Leech, Mouse, Rabbit, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids COVID-19 Apoptosis Cancer Cell Culture Immune System Inflammation Stem Cells & Differentiation Allergy Transplantation Recent Citations First Author George, S Title Protocol to drive human monocyte-to-macrophage polarization in vitro using tumor conditioned media. Citation STAR Protocols; 3(4) pg101666 PubMed ID 36125932 First Author Li, Y Title Targeted inhibition of STAT3 induces immunogenic cell death of hepatocellular carcinoma cells via glycolysis. Citation Molecular Oncology; 16(15) pg2861-2880 PubMed ID 35665592 First Author Loef, E J Title Plasminogen and plasmin can bind to human T cells and generate truncated CCL21 that increases dendritic cell chemotactic responses. Citation The Journal of Biological Chemistry; 298(7) pg102112 PubMed ID 35690148 Product Reviews Existing reviews hIL4 Good product. Used for in vitro culture to derive BMDCs. From: Kevin | Date: 26/08/2020 × hIL4 Good product. Used for in vitro culture to derive BMDCs. From: Kevin | Date: 26/08/2020 Close Great for APCs Used to differentiate APCs, works very consistently From: Karma | Date: 26/08/2020 × Great for APCs Used to differentiate APCs, works very consistently From: Karma | Date: 26/08/2020 Close MDSCs Used to differentiate PBMCs into MDSCs. Works well. From: Max | Date: 27/08/2020 × MDSCs Used to differentiate PBMCs into MDSCs. Works well. From: Max | Date: 27/08/2020 Close
