Animal-Free Cytokines

Animal-Free Recombinant Human M-CSF

Product Details

Catalogue Number: AF-300-25
Description:

M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. Human M-CSF is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant Human M-CSF is a 36.8 kDa homodimeric protein consisting of two 159 amino acid polypeptide subunits.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Macrophage Colony Stimulating Factor, CSF-1, MGI-IM

AA Sequence (monomer): MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 36.8 kDa

Accession Number: P09603

Gene ID: 1435

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Meng, Q
Title
Reversible ubiquitination shapes NLRC5 function and modulates NF-κB activation switch.
Citation
The Journal of Cell Biology; 211(5) pg1025-40
PubMed Id
First Author
Rossato, M
Title
IL-10-induced microRNA-187 negatively regulates TNF-α, IL-6, and IL-12p40 production in TLR4-stimulated monocytes.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 109(45) pgE3101-10
PubMed Id
First Author
Beavis, P A
Title
Resistance to regulatory T cell-mediated suppression in rheumatoid arthritis can be bypassed by ectopic foxp3 expression in pathogenic synovial T cells.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 108(40) pg16717-22
PubMed Id

product.subtitle.productreviews

Existing reviews
Very good experience
We use M-CSF to differentiate human and rabbit macrophages and we can highly recommend it.
From:  Hanna-Mari | Date:  19/08/2020
Human MCSF
I am working on bone resorption model and I was looking for key growth factors induce osteoclastic differentiation. I purchased the product from Peprotech. I was pretty impressed by the product, technical assistance from the staff and also the protocol on how to reconstitute the product to yield usable working solution. I am having good experience with Peprotech. Hope to see you provide this suppo... reviews.showmore
From:  Rajeshwari | Date:  27/08/2020