Animal-Free Recombinant Human M-CSF 2 ReviewsSubmit a Review Product Details Catalog Number: AF-300-25 Description: M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. Human M-CSF is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant Human M-CSF is a 36.8 kDa homodimeric protein consisting of two 159 amino acid polypeptide subunits. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Macrophage Colony Stimulating Factor, CSF-1, MGI-IM AA Sequence (monomer): MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 36.8 kDa Accession Number: P09603 Gene ID: 1435 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) Cross Reactivity Cited in References: Human, Monkey, Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cancer Immune System Stem Cells & Differentiation Recent Citations First Author Laucirica, D R Title Pseudomonas aeruginosa modulates neutrophil granule exocytosis in an in vitro model of airway infection. Citation Immunology and Cell Biology; 100(5) pg352-370 PubMed ID 35318736 First Author Murthy, S Title Danger signal extracellular calcium initiates differentiation of monocytes into SPP1/osteopontin-producing macrophages. Citation Cell death & disease; 13(1) pg53 PubMed ID 35022393 First Author Geuijen, C Title A human CD137×PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade Citation Nature Communications; 12(1) pg4445 PubMed ID 34290245 Product Reviews Existing reviews Very good experience We use M-CSF to differentiate human and rabbit macrophages and we can highly recommend it. From: Hanna-Mari | Date: 19/08/2020 × Very good experience We use M-CSF to differentiate human and rabbit macrophages and we can highly recommend it. From: Hanna-Mari | Date: 19/08/2020 Close Human MCSF I am working on bone resorption model and I was looking for key growth factors induce osteoclastic differentiation. I purchased the product from Peprotech. I was pretty impressed by the product, technical assistance from the staff and also the protocol on how to reconstitute the product to yield usable working solution. I am having good experience with Peprotech. Hope to see you provide this suppo... Show More From: Rajeshwari | Date: 27/08/2020 × Human MCSF I am working on bone resorption model and I was looking for key growth factors induce osteoclastic differentiation. I purchased the product from Peprotech. I was pretty impressed by the product, technical assistance from the staff and also the protocol on how to reconstitute the product to yield usable working solution. I am having good experience with Peprotech. Hope to see you provide this support to the scientific field constantly.Thank you,Rajeshwari HR From: Rajeshwari | Date: 27/08/2020 Close
