Animal-Free Cytokines

Animal-Free Recombinant Human/Murine/Rat Activin A (E.coli derived)

Product Details

Catalogue Number: AF-120-14E
Description:

Activin A is a TGF-β family member that exhibits a wide range of biological activities, including regulation of cellular proliferation and differentiation, and promotion of neuronal survival.  Elevated levels of Activin A in human colorectal tumors and in postmenopausal women have been implicated in colorectal and breast cancers, respectively.  The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-β antagonist, follistatin.  Activin A binds to the two forms of activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B).  Activins are homodimers or heterodimers of different β subunits.  They are produced as precursor proteins with an amino terminal propeptide that is cleaved to release the C-terminal bioactive ligand.  Recombinant Human/Murine/Rat Activin A is a 26.0 kDa disulfide-linked homodimer of two βA chains, each containing 116 amino acid residues.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Inhibin beta-1, FRP, FSH (Follicle-stimulating hormone)-releasing protein, Erythroid Differentiation Factor (EDF)

AA Sequence (monomer): GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

Purity: ≥ 97% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 26 kDa

Accession Number: P08476

Gene ID: 3624

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

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First Author
Sugishita, H
Title
Variant PCGF1-PRC1 links PRC2 recruitment with differentiation-associated transcriptional inactivation at target genes
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Nature Communications; 12(1) pg5341
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Watanabe, A
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CD82 is a marker to isolate β cell precursors from human iPS cells and plays a role for the maturation of β cells
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Scientific Reports; 11(1) pg9530
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Griffiths, R
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The Transcription Factor-microRNA Regulatory Network during hESC-chondrogenesis
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Scientific Reports; 10(1) pg4744
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