Recombinant Human Osteopontin 0 ReviewsSubmit a Review Product Details Catalogue Number: 120-35 Description: Osteopontin is a secreted glycoprotein that functions as a ligand to αvβ3 integrin and possibly other receptors. It binds tightly to hydroxyapatite and can act as a structural component of the extracellular mineralized matrix. Osteopontin is initially secreted as a 298 amino acid protein, which is subject to multiple post-translational modifications including glycosylation, phosphorylation, and specific proteolytic cleavages into various smaller molecular weight fragments. Osteopontin is expressed in a wide range of cells and tissues including osteoblasts, various tumor cell lines, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta and odontoblasts. In addition to its involvement in mineralized matrix formation, Osteopontin can also function as a cytokine that stimulates the release of IFNγ and IL-12, while inhibiting the production of IL-10. Recombinant Human Osteopontin is a 298 amino acid protein, which, due to glycosylation, migrates at an apparent molecular weight of 60.0-65.0 kDa by SDS-PAGE analysis under reducing conditions. Source: HEK293 cells Synonyms: Bone sialoprotein 1, Secreted phosphoprotein 1, SPP-1, Nephropontin, Uropontin AA Sequence: IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN Purity: ≥ 97% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to enhance cell adhesion of murine B16-F1 cells. Calculated Molecular Weight: 33.7 kDa Accession Number: P10451 Gene ID: 6696 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Bone, Skeletal, Cartilage Cancer product.subtitle.recentcitations First Author MacDonald, L Title COVID-19 and RA share an SPP1 myeloid pathway that drives PD-L1+ neutrophils and CD14+ monocytes. Citation JCI Insight; 6(13) PubMed Id 34143756 First Author Yang, Y Title Squalene synthase promotes the invasion of lung cancer cells via the osteopontin/ERK pathway Citation Oncogenesis; 9(8) pg78 PubMed Id 32862200 First Author Zhang, L Title Single-Cell Analyses Inform Mechanisms of Myeloid-Targeted Therapies in Colon Cancer. Citation Cell; 181(2) pg442-459.e29 PubMed Id 32302573