Recombinant Human R-Spondin-1 4 ReviewsSubmit a Review Product Details Catalog Number: 120-38 Description: R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in the adrenal glands, ovary, testis, thyroid, and trachea. Rspo can interact with the Frizzled/LRP6 receptor complex in a manner that stimulates the Wnt/β-catenin signaling pathway. Recombinant Human R-Spondin-1 is a 26.7 kDa protein consisting of 243 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS-PAGE analysis under reducing conditions. Source: CHO cells Synonyms: Roof plate-specific spondin-1, RSPO1 AA Sequence: SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses. Biological Activity: R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The expected ED50 is 1.0-3.0 μg/ml. Calculated Molecular Weight: 26.7 kDa Accession Number: Q2MKA7 Gene ID: 284654 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Note: 1mg will be provided as 2x500μg. Cross Reactivity Cited in References: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Cancer Neurobiology Stem Cells & Differentiation Recent Citations First Author Yoon, Y Title Salivary gland organoid culture maintains distinct glandular properties of murine and human major salivary glands. Citation Nature Communications; 13(1) pg3291 PubMed ID 35672412 First Author Ryu, T Y Title Human gut-microbiome-derived propionate coordinates proteasomal degradation via HECTD2 upregulation to target EHMT2 in colorectal cancer. Citation The ISME Journal; 16(5) pg1205-1221 PubMed ID 34972816 First Author Buchanan, K L Title The preference for sugar over sweetener depends on a gut sensor cell. Citation Nature Neuroscience; 25(2) pg191-200 PubMed ID 35027761 Product Reviews Existing reviews Perfect for organoid culture My lung organoids grow well consistently even after passaging it couple of times and there were no contamination issues. From: Eun Joo | Date: 08/07/2020 × Perfect for organoid culture My lung organoids grow well consistently even after passaging it couple of times and there were no contamination issues. From: Eun Joo | Date: 08/07/2020 Close Good for organoid This is good for my primary lung organoid culture. From: Jun | Date: 21/07/2020 × Good for organoid This is good for my primary lung organoid culture. From: Jun | Date: 21/07/2020 Close High quality and good price This product worked well for my project. The price is reasonable and lower than most of vendors. From: Jin | Date: 26/08/2020 × High quality and good price This product worked well for my project. The price is reasonable and lower than most of vendors. From: Jin | Date: 26/08/2020 Close Excellent qualiity We have been using recombinant human R-spondin-1 for over 6 years and have been absolutely satisfied with the quality, purity, and performance! From: Candace | Date: 03/08/2021 × Excellent qualiity We have been using recombinant human R-spondin-1 for over 6 years and have been absolutely satisfied with the quality, purity, and performance! From: Candace | Date: 03/08/2021 Close
