Recombinant Human sCD14 0 ReviewsSubmit a Review Product Details Catalogue Number: 110-01 Description: CD14 is a cell surface-anchored glycoprotein that is expressed predominantly by monocytes and tissue macrophages. CD14 associates with MD-2 (LY-96) and TLR4 to form a receptor complex, which signals specifically in response to bacterial lipopolysaccharide (LPS) binding. The CD14/MD-2/TLR4 receptor complex signals via MyD88, TIRAP, and TRAF6, and ultimately activates NF-κB. CD14 also exists in a soluble form, designated as sCD14, which is capable of specifically binding LPS in the extracellular space. Recombinant Human sCD14 is a 331 amino acid glycoprotein comprising the extracellular portion of the CD14 receptor. The calculated molecular weight of Recombinant Human sCD14 is 35.6 kDa. Source: HEK293 cells Synonyms: soluble CD14, Monocyte differentiation antigen CD14 AA Sequence: TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by the dose-dependent activation of NF-κB in a RAW264 cell line based reporter system, using a sCD14 concentration range of 20 ng/μl to 200 ng/μl. The NF-κB activation is enhanced when the assay is done in the presence of 0.25 ng/μl to 1.0 ng/μl bacterial LPS. Calculated Molecular Weight: 35.6 kDa Accession Number: P08571 Gene ID: 929 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Mouse, References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Immune System Inflammation Receptors product.subtitle.recentcitations First Author Saravanan, R Title Structural basis for endotoxin neutralisation and anti-inflammatory activity of thrombin-derived C-terminal peptides. Citation Nature Communications; 9(1) pg2762 PubMed Id 30018388 First Author Andrukhov, O Title Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists. Citation Scientific Reports; 7(1) pg12856 PubMed Id 28993635 First Author Hu, L Title Extraordinarily potent proinflammatory properties of lactoferrin-containing immunocomplexes against human monocytes and macrophages. Citation Scientific Reports; 7(1) pg4230 PubMed Id 28652573
