Recombinant Human sTRAIL/Apo2L 0 ReviewsSubmit a Review Product Details Catalog Number: 310-04 Description: TRAIL/Apo2L is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins, and contain a cytoplasmic "death domain", which activates the cell's apoptotic machinery. The full length human TRAIL/Apo2L is a 281 amino acid protein, consisting of a 17 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 243 amino acid extracellular domain. Recombinant Human soluble TRAIL/Apo2L is a 168 amino acid polypeptide (19.6 kDa), consisting of the TNF-homologous portion of the extracellular domain of the full length TRAIL/Apo2L protein. Source: E.coli Synonyms: TNF-Related Apoptosis-Inducing Ligand, TNFSF10, Apo2 Ligand, TL2 AA Sequence: MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Assay #1: Determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/ml. Assay #2: Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 0.8-2.0 ng/ml. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 19.6 kDa Accession Number: P50591 Gene ID: 8743 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Cross Reactivity Cited in References: Bacteria, Chicken, Human, Mouse, References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Apoptosis Cancer Immune System TNF Superfamily Recent Citations First Author Ishii, Y Title BAP1 and YY1 regulate expression of death receptors in malignant pleural mesothelioma. Citation The Journal of Biological Chemistry; pg101223 PubMed ID 34597666 First Author Locquet, M Title Caspase-8 deficiency induces a switch from TLR3 induced apoptosis to lysosomal cell death in neuroblastoma. Citation Scientific Reports; 11(1) pg10609 PubMed ID 34011952 First Author Jiang, Y Title O-glycans on death receptors in cells modulate their sensitivity to TRAIL-induced apoptosis through affecting on their stability and oligomerization. Citation FASEB journal : official publication of the Federation of American Societies for Experimental Biology; 34(9) pg11786-11801 PubMed ID 32692906
