Growth Factors & Cytokines

Recombinant Human TGF-β1 (CHO derived)

Product Details

Catalogue Number: 100-21C
Description:

The three mammalian isoforms of TGF-β, TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses.  They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well as synthesis and deposition of the extracellular matrix.  They are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing.  They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix.  The release of the biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1.  TGF-β1 is the most abundant isoform secreted by almost every cell type.  It was originally identified for its ability to induce phenotypic transformation of fibroblasts, and recently it has been implicated in the formation of skin tumors.  Recombinant Human TGF-β1 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

Source: CHO cells

Synonyms: Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor

AA Sequence (monomer): ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by TGF-β1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg

Calculated Molecular Weight: 25 kDa

Accession Number: P01137

Gene ID: 7040

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

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Existing reviews
Best product for smooth muscle differentiation
We have used this product to promote both human ESC and iPSCs-derived smooth muscle cell differentiation for more than 3 years and obtain solid data for more than four publication recently. The price is much lower than other companies. We trust this company by ordering most cytokines for our stem cells/cardiovascular disease modeling projects.
From:  Ping | Date:  08/07/2020
Great reliable cytokine to make CD4+ Tregs
We have been using this cytokine for years now when we differentiate either mouse CD4 Tregs or Th17s and also human Treg cells. The pricing is also very reasonable when sized up.
From:  Chirag | Date:  31/08/2020
Great product with high quality and affordable price
We have used this product for in vitro smooth muscle cell differentiation from human IPSCs for more than 4 years, which contributes to several high quality publication  such as Circulation, ATVB, and Nature Comm. Compare to other's suppliers product, this one has high quality as well as reasonable price
From:  Ping | Date:  03/08/2021