Recombinant Human TWEAK 0 ReviewsSubmit a Review Product Details Catalogue Number: 310-06 Description: TWEAK belongs to the TNF family of ligands, and signals through TWEAKR, also known as TNFRSF12A. TWEAK is expressed in a variety of tissues, including the adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK has the ability to induce NF-κB activation and chemokine secretion, and to exert an apoptotic activity in certain cells, such as HT-29 human adenocarcinoma cells when cultured in the presence of IFN-γ. TWEAK also promotes proliferation and migration of endothelial cells. The human TWEAK gene encodes for a 249 amino acid type II transmembrane protein, which contains a 21 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 207 amino acid extracellular domain. Recombinant Human TWEAK is a soluble 17.0 kDa polypeptide (154 amino acid residues) comprising the TNF-homologous region of TWEAK, and is generated by proteolytic processing of the full length membrane-anchored TWEAK protein. Source: E.coli Synonyms: TNF-related weak inducer of apoptosis, TNFSF12, DR3LG, Apo3 Ligand AA Sequence: MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Assay #1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC is less than 10 ng/ml. Assay #2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-γ. The ED50 for this effect is between 30-45 ng/ml. Calculated Molecular Weight: 17 kDa Accession Number: O43508 Gene ID: 8742 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Apoptosis Immune System Inflammation Neurobiology TNF Superfamily product.subtitle.recentcitations First Author Vredevoogd, D Title Augmenting Immunotherapy Impact by Lowering Tumor TNF Cytotoxicity Threshold. Citation Cell; 178(3) pg585-599.e15 PubMed Id 31303383 First Author Ramakrishnan, S Title Intestinal non-canonical NFκB signaling shapes the local and systemic immune response. Citation Nature Communications; 10(1) pg660 PubMed Id 30737385 First Author Winer, H Title Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins. Citation Nature Communications; 9(1) pg3744 PubMed Id 30218067
