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Growth Factors & Cytokines

Recombinant Human VEGF165

Product Details

Catalogue Number: 100-20
Description:
Recombinant Human VEGF 165

VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant Human VEGF165 is a 38.2 kDa, disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.

Source: E.coli

Synonyms: Vascular Endothelial Growth Factor, VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, VEGF-A

AA Sequence (monomer): APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-8.0 ng/ml.

This product has been tested against the WHO standard. International Unit information can be found here.

Recombinant Human VEGF 165 WHO Comparison Graph Recombinant Human VEGF 165 WHO Comparison Graph

Calculated Molecular Weight: 38.2 kDa

Accession Number: P15692

Gene ID: 7422

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Kitajima, K
Title
An Interferon-γ/FLT3 Axis Positively Regulates Hematopoietic Progenitor Cell Expansion from Human Pluripotent Stem Cells.
Citation
Stem cells (Dayton, Ohio); 40(10) pg906-918
PubMed Id
First Author
Dhami, S P
Title
Breast cancer cells mediate endothelial cell activation, promoting von Willebrand factor release, tumor adhesion, and transendothelial migration.
Citation
Journal Of Thrombosis And Haemostasis : JTH; 20(10) pg2350-2365
PubMed Id
First Author
Hyun, J
Title
Notch1 promotes ordered revascularization through Semaphorin 3g modulation of downstream vascular patterning signalling factors.
Citation
The Journal of Physiology; 600(3) pg509-530
PubMed Id

product.subtitle.productreviews

Existing reviews
Great for Endothelial Cell Differentiation
This is an excellent product with long-term stability if is stored correctly. I have been regularly using this product since 2017 for endothelial cell differentiation from human induced pluripotent cells (iPSCs). Thanks PeproTech for consistent quality product!
From:  DIlip | Date:  13/07/2020
Excellent product for endothelial cells culture
I work since 2019 with human iPSC-derived endothelial cells, and the recombinant human VEGF 165 is mandatory for maintaining them in culture. VEGF 165 is an excellent product, it is stable for a long storage, easy to prepare and to use.  Always receive it in time.I recommend this product.  Thank you, PeproTech.
From:  Amal | Date:  18/08/2021