Extracellular Matrix

Animal-Free Recombinant Human Vitronectin

Product Details

Catalogue Number: AF-140-09
Description:

Vitronectin is a secreted glycoprotein that is synthesized in the liver. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide-linked multimers. The multimeric vitronectin can efficiently bind to, and incorporate into, the extracellular matrix. Within the matrix, vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Additionally, recombinant vitronectin can function as a chemically-defined matrix component in human embryonic stem cell renewal media. Recombinant Human Vitronectin is a 459 amino acid, single-chain, monomeric protein, which migrates at an apparent molecular weight of 75 kDa by SDS-PAGE under reducing conditions. The calculated molecular weight of Recombinant Human Vitronectin is 52.2 kDa.

Manufactured using all Animal-Free reagents.

Source: HEK293 cells

Synonyms: VTN, Serum-spreading factor, V75

AA Sequence: DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Calculated Molecular Weight: 52.2 kDa

Biological Activity: Recombinant human Vitronectin promotes attachment of hESC and iPSC in serum-free, feeder conditions at 5ug/ml. See PeproTech’s hESC medium manual for further information.

Accession Number: P04004

Gene ID: 7448

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Walsh, P
Title
Accelerated differentiation of human pluripotent stem cells into neural lineages via an early intermediate ectoderm population.
Citation
Stem cells (Dayton, Ohio);
PubMed Id
First Author
Kawamura, M
Title
Induction of migration of periodontal ligament cells by selective regulation of integrin subunits.
Citation
Journal of Cellular and Molecular Medicine; 23(2) pg1211-1223
PubMed Id
First Author
Geng, Z
Title
Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration.
Citation
PLoS ONE; 12(3) pge0173575
PubMed Id