Recombinant Human/Murine/Rat BMP-2 (CHO derived) 1 ReviewsSubmit a Review Product Details Catalogue Number: 120-02C Description: BMPs (Bone Morphogenetic Proteins) belong to the TGF-β superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with an osteoconductive carrier such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 appears to play an important role in cardiac morphogenesis, and is expressed in a variety of other tissues, including lung, liver, spleen, prostate, ovary, and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains (monomers) linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease. Recombinant Human/Murine/Rat BMP-2 derived from CHO cells is a homodimeric glycoprotein that consists of two 114 amino acid polypeptide chains linked by a single disulfide bond. Due to glycosylation, CHO cell-derived Human/Murine/Rat BMP-2 migrates at an apparent molecular weight of approximately 28-29 kDa by SDS-PAGE analysis under non-reducing conditions. Source: CHO cells Synonyms: Bone Morphogenetic Protein-2, BMP-2A AA Sequence (monomer): QAKHKQRKRL KSSCKRHPLY VDFSDVGWND WIVAPPGYHA FYCHGECPFP LADHLNSTNH AIVQTLVNSV NSKIPKACCV PTELSAISML YLDENEKVVL KNYQDMVVEG CGCR Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 40-100 ng/ml. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 25.8 kDa Accession Number: P12643 Gene ID: 650 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Mouse, References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Apoptosis Bone, Skeletal, Cartilage Cell Culture Immune System Neurobiology Stem Cells & Differentiation TGF-β Superfamily product.subtitle.recentcitations First Author Colucci, S Title Liver Sinusoidal Endothelial Cells Suppress Bone Morphogenetic Protein 2 Production in Response to TGFβ Pathway Activation. Citation Hepatology (Baltimore, Md.); 74(4) pg2186-2200 PubMed Id 33982327 First Author Guo, D Title Tumor cells generate astrocyte-like cells that contribute to SHH-driven medulloblastoma relapse. Citation The Journal of Experimental Medicine; 218(9) PubMed Id 34254999 First Author Han, Y Title Identification of SARS-CoV-2 inhibitors using lung and colonic organoids. Citation Nature; 589(7841) pg270-275 PubMed Id 33116299 product.subtitle.productreviews Existing reviews Quality Product BMP-2 used for mesenchymal differentiation. Very cost effective. Next day delivery, Thanks. From: Hardik | Date: 14/08/2020 × Quality Product BMP-2 used for mesenchymal differentiation. Very cost effective. Next day delivery, Thanks. From: Hardik | Date: 14/08/2020 Close