Growth Factors & Cytokines

Recombinant Human/Murine/Rat BMP-2 (E.coli derived)

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Product Details

Catalogue Number: 120-02
Description:


Recombinant Human/Murine/Rat BMP-2 (E.coli derived)

BMPs (Bone Morphogenetic Proteins) belong to the TGF-β superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis, and is expressed in a variety of tissues, including lung, spleen, brain, liver, prostate, ovary, and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease. Recombinant Human/Murine/Rat BMP-2 is a 26.0 kDa homodimeric protein consisting of two 115 amino acid polypeptide chains.

Source: E.coli

Synonyms: Bone Morphogenetic Protein-2, BMP-2A

AA Sequence (monomer): MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.0 μg/ml.

Calculated Molecular Weight: 26 kDa

Accession Number: P12643

Gene ID: 650

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Remšík, J
Title
TGF-β regulates Sca-1 expression and plasticity of pre-neoplastic mammary epithelial stem cells
Citation
Scientific Reports; 10(1) pg11396
PubMed Id
First Author
Abu-Bonsrah, K D
Title
Generation of Adrenal Chromaffin-like Cells from Human Pluripotent Stem Cells.
Citation
Stem Cell Reports; 10(1) pg134-150
PubMed Id
First Author
Dupont, S
Title
FAM/USP9x, a deubiquitinating enzyme essential for TGFbeta signaling, controls Smad4 monoubiquitination.
Citation
Cell; 136(1) pg123-35
PubMed Id