Recombinant Murine Granzyme B 0 ReviewsSubmit a Review Product Details Catalog Number: 140-03 Description: Granzyme B is a cysteine protease found in the cytoplasmic granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B is required for the induction of target cell lysis, which occurs as part of cell-mediated immune responses, and can activate apoptosis in target cells by both caspase-dependent and caspase-independent mechanisms. Proteolytic cleavage of substrates by Granzyme B takes place primarily after aspartic acid residues. Recombinant Murine Granzyme B is a glycosylated 227 amino acid protein, comprising the mature active portion of the murine Granzyme B precursor. The apparent molecular weight is 28.9 kDa by mass spectrometry. Source: Baculovirus Synonyms: Cytotoxic cell protease 1 (CCP1) AA Sequence: IIGGHEVKPH SRPYMALLSI KDQQPEAICG GFLIREDFVL TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM APMGKYSNTL QEVELTVQKD RECESYFKNR YNKTNQICAG DPKTKRASFR GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI KKTMKSS Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 25°C, is greater than 750 nM/min per µg of enzyme. Calculated Molecular Weight: 28.9 kDa Accession Number: P04187 Gene ID: 14939 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Note: 100μg is the largest vial size for this product. Larger sizes supplied as multiple 100μg vials. Cross Reactivity Cited in References: Mouse, References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Apoptosis Immune System Inflammation Transplantation Recent Citations First Author Holt, B A Title Protease circuits for processing biological information. Citation Nature Communications; 11(1) pg5021 PubMed ID 33024117 First Author Gu, H Title Gambogic acid induced tumor cell apoptosis by T lymphocyte activation in H22 transplanted mice. Citation International Immunopharmacology; 8(11) pg1493-502 PubMed ID 18573352
