Growth Factors & Cytokines

Recombinant Murine LIF

Product Details

Catalogue Number: 250-02
Description:

LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Murine LIF is a 19.9 kDa protein containing 180 amino acids residues, including three disulfide bonds.

Source: E.coli

Synonyms: Leukemia Inhibitory Factor, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLPLI), Interleukin 6 family cytokine

AA Sequence: SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: PeproTech's Murine LIF is fully biologically active when compared to standards. The ED50 as determined by the M1 cell differentiation assay is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

Calculated Molecular Weight: 19.6 kDa

Accession Number: P09056

Gene ID: 16878

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Zhu, Y
Title
Cell cycle heterogeneity directs spontaneous 2C state entry and exit in mouse embryonic stem cells.
Citation
Stem Cell Reports; 16(11) pg2659-2673
PubMed Id
First Author
Wulansari, N
Title
LIF maintains mouse embryonic stem cells pluripotency by modulating TET1 and JMJD2 activity in a JAK2-dependent manner.
Citation
Stem cells (Dayton, Ohio); 39(6) pg750-760
PubMed Id
First Author
Panda, S
Title
IL-4 controls activated neutrophil FcγR2b expression and migration into inflamed joints.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 117(6) pg3103-3113
PubMed Id