Growth Factors & Cytokines

Recombinant Murine M-CSF

Product Details

Catalogue Number: 315-02
Description:

M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. Human M-CSF is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant Murine M-CSF is a 36.4 kDa homodimeric protein consisting of two 156 amino acid polypeptide subunits.

Source: E.coli

Synonyms: Macrophage Colony Stimulating Factor, CSF-1, MGI-IM

AA Sequence (monomer): MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of M-NFS-60 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

Calculated Molecular Weight: 36.4 kDa

Accession Number: P07141

Gene ID: 12977

crossreactivity:
Country Of Origin: USA

Not for human use.

Country Of Origin: USA

Not for human use.

product.subtitle.productreviews

Existing reviews
Differencitaion of bone marrow progenitors in bone marrow derived macrophages
I used murine M-CSF to differentiate bone marrow progenitors in bone marrow derived macrophages.
From:  Laura | Date:  28/07/2020
MCSF
Good product. Works well to generate macrophages from BM.
From:  Kevin | Date:  26/08/2020
Excellent product
I have used this product for differentiating bone marrow-derived monocytes to macrophages. It works very well based on visual observations as well as flow cytometry data on macrophage surface protein expression. I use it very often for my project.
From:  Kiran | Date:  26/08/2020
M-CSF for maintaining mouse microglial lines
I have been using murine M-CSF for maintaining mouse microglial lines, EOC20. And it worked perfectly. Definitely  recommend.
From:  Yongjin | Date:  26/08/2020