Growth Factors & Cytokines

Recombinant Murine Noggin

Product Details

Catalogue Number: 250-38
Description:
Recombinant Murine Noggin

Noggin belongs to a group of diffusible proteins that bind to ligands of the TGF-β family, and regulate their activity by inhibiting their access to signaling receptors.  Noggin was originally identified as a BMP-4 antagonist whose action was critical for proper formation of the head and other dorsal structures.  Consequently, noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14.  Targeted deletion of noggin in mice results in prenatal death, and a recessive phenotype displaying a severely malformed skeletal system.  Conversely, transgenic mice over-expressing noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.  Recombinant Murine Noggin is a 46.4 kDa disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains.

Source: E.coli

Synonyms: None

AA Sequence (monomer): MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC5 chondrogenic cells. The expected ED50 for this effect is 1.0-2.0 ng/ml of Noggin.

Calculated Molecular Weight: 46.4 kDa

Accession Number: P97466

Gene ID: 18121

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Drurey, C
Title
Intestinal epithelial tuft cell induction is negated by a murine helminth and its secreted products.
Citation
The Journal of Experimental Medicine; 219(1)
PubMed Id
First Author
Zhang, S
Title
Long noncoding RNA Meg3 sponges miR-708 to inhibit intestinal tumorigenesis via SOCS3-repressed cancer stem cells growth.
Citation
Cell death & disease; 13(1) pg25
PubMed Id
First Author
Belenguer, G
Title
RNF43/ZNRF3 loss predisposes to hepatocellular-carcinoma by impairing liver regeneration and altering the liver lipid metabolic ground-state.
Citation
Nature Communications; 13(1) pg334
PubMed Id

product.subtitle.productreviews

Existing reviews
organoids
Works perfect with both human and murine pancreatic organoids.
From:  Mitesh | Date:  09/07/2020
Murine Noggin for CRC and pan Organoid Culture
Murine Noggin I am using for Human as well as mouse Pancreas, Colon and Rectal Organoid culture medias.
Consistent activity each lot and next day delivery.
From:  Hardik | Date:  14/08/2020
mNoggin for organoids culture
We have used it for both mouse and human pancreatic organoids and it works perfectly!
From:  Pilar | Date:  27/08/2020