Animal-Free Recombinant Murine SCF 1 ReviewsSubmit a Review Product Details Catalog Number: AF-250-03 Description: SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. The human SCF gene encodes for a 273 amino acid transmembrane protein, which contains a 25 amino acid N-terminal signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. The secreted soluble form of SCF is generated by proteolytic processing of the membrane anchored precursor. Recombinant Murine SCF is an 18.3 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor AA Sequence: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 10.0 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg. Calculated Molecular Weight: 18.3 kDa Accession Number: P20826 Gene ID: 17311 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) Cross Reactivity Cited in References: Human, Monkey, Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cell Culture Immune System Stem Cells & Differentiation Recent Citations First Author Zhang, M Title The transcription factor TFCP2L1 promotes primordial germ cell-like cell specification of pluripotent stem cells. Citation The Journal of Biological Chemistry; pg101217 PubMed ID 34555410 First Author Ambrosi, T H Title Aged skeletal stem cells generate an inflammatory degenerative niche Citation Nature; 597(7875) pg256-262 PubMed ID 34381212 First Author Nguyen, G T Title Neutrophils require SKAP2 for reactive oxygen species production following C-type lectin and Candida stimulation. Citation Iscience; 24(8) pg102871 PubMed ID 34386732 Product Reviews Existing reviews Animal-Free Recombinant Murine SCF Great product, works well! From: Petra | Date: 03/08/2021 × Animal-Free Recombinant Murine SCF Great product, works well! From: Petra | Date: 03/08/2021 Close
