Produced from sera of rabbits pre-immunized with highly pure (>98%)
recombinant hBD-5. Anti-Human BD-5 specific antibody was purified by
affinity chromatography employing immobilized hBD-5 matrix.
E.coli derived Recombinant Human BD-5 (PeproTech catalog# 300-68)
To detect hBD-5 by sandwich ELISA (using 100 μl/well antibody solution) a
concentration of 0.5 - 2.0 μg/ml of this antibody is required. This
antigen affinity purified antibody, in conjunction with PeproTech’s
Biotinylated Anti-Human BD-5 (500-P323Bt) as a detection antibody, allows
the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-5.
To detect hBD-5 by Western Blot analysis this antibody can be used at a
concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible
secondary reagents the detection limit for recombinant hBD-5 is 1.5-3.0
ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.