Produced in BALB/c x ICR F1 mice using highly pure recombinant human FGF-basic as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by Protein A affinity chromatography.
E.coli derived Recombinant Human FGF-basic (154 a.a.) (PeproTech catalog# 100-18B)
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human FGF-Basic when used with PeproTech's Biotinylated Antigen Affinity Purified Anti-Human FGF-Basic (500-P18BT) as the detection antibody at a concentration of approximately 0.125-0.25 μg/ml.
To detect Human FGF-Basic by Western Blot analysis this antibody can be used at a concentration of 0.25- 0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human FGF-Basic is 1.0-2.0 ng/lane, under non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.