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Monoclonal Antibodies

Anti-Human IL-2 (Monoclonal Mouse)

제품 상세

카다로그 번호 500-M02

Source: Monoclonal Mouse

Preparation: Produced in mice immunized with highly pure Recombinant Human IL-2. Anti-Human IL-2-specific antibody was purified from cell culture by Protein A affinity chromatography.

Immunogen: E.coli derived Recombinant Human IL-2 (PeproTech catalog# 200-02)

Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-2 when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human IL-2 (500-P22BT) as the detection antibody at a concentration of approximately 1.0- 2.0 μg/ml.

Anti-Human IL-2 Sandwich ELISA

Western Blot: To detect Human IL-2 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.

Anti-Human IL-2 Western Blot Reduced Anti-Human IL-2 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity: Research Interest

Country Of Origin: USA

Not for human use.

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기존 리뷰
Worked on Cultured PBMCs
Had a great experience using this cytokine with cultured PBMCs. Used it at 10ng/mL and saw good viability and downstream response to stimulation with PMA/Ionomycin.
에서:  Etienne | 날짜:  2020-07-13
Worked on Cultured PBMCs
Had a great experience using this cytokine with cultured PBMCs. Used it at 10ng/mL and saw good viability and downstream response to stimulation with PMA/Ionomycin.
에서:  Etienne | 날짜:  2020-07-13