Biotinylated Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat EGF (Rat Epidermal Growth Factor). Anti-Rat EGF specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Rat EGF (PeproTech catalog# 400-25)

Sandwich ELISA: To detect Rat EGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Rat EGF (500-P277) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat EGF.

Western Blot: To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

1mg will be provided as 2x500μg.