Recombinant Human MMP-1 0 ReviewsSubmit a Review Product Details Catalog Number: 420-01 Description: Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-1 is a secreted collagenase with specificity toward Type I, II, III, VII, and X collagens. Recombinant Human MMP-1 is a 42.7 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain, which is involved in substrate specificity, and in binding TIMP-1. Source: E.coli Synonyms: Matrix Metalloproteinase-1, Fibroblast Collagenase, Interstitial Collagenase AA Sequence: MFVLTEGNPR WEQTHLTYRI ENYTPDLPRA DVDHAIEKAF QLWSNVTPLT FTKVSEGQAD IMISFVRGDH RDNSPFDGPG GNLAHAFQPG PGIGGDAHFD EDERWTNNFR EYNLHRVAAH ELGHSLGLSH STDIGALMYP SYTFSGDVQL AQDDIDGIQA IYGRSQNPVQ PIGPQTPKAC DSKLTFDAIT TIRGEVMFFK DRFYMRTNPF YPEVELNFIS VFWPQLPNGL EAAYEFADRD EVRFFKGNKY WAVQGQNVLH GYPKDIYSSF GFPRTVKHID AALSEENTGK TYFFVANKYW RYDEYKRSMD PGYPKMIAHD FPGIGHKVDA VFMKDGFFYF FHGTRQYKFD PKTKRILTLQ KANSWFNCRK N Purity: ≥ 90% by SDS-PAGE gel and HPLC analyses. Biological Activity: MMP-1 activity was measured by its ability to cleave a chromogenic peptide MMP-1 substrate at room temperature. At an MMP-1 concentration of 2.5 μg/ml, 50% cleavage was achieved at an incubation time of approximately 25 minutes. Calculated Molecular Weight: 42.7 kDa Accession Number: P03956 Gene ID: 4312 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Note: 1mg will be provided as 2x500μg. Cross Reactivity Cited in References: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Bone, Skeletal, Cartilage Cancer Immune System Inflammation Wound Healing Recent Citations First Author Lienemann, P S Title Smart Hydrogels for the Augmentation of Bone Regeneration by Endogenous Mesenchymal Progenitor Cell Recruitment. Citation Advanced science (Weinheim, Baden-Wurttemberg, Germany); 7(7) pg1903395 PubMed ID 32274319 First Author Wu, K Title Roles of the cyclooxygenase 2 matrix metalloproteinase 1 pathway in brain metastasis of breast cancer. Citation The Journal of Biological Chemistry; 290(15) pg9842-54 PubMed ID 25691572 First Author Przybyla, L M Title Matrix remodeling maintains embryonic stem cell self-renewal by activating Stat3. Citation Stem cells (Dayton, Ohio); 31(6) pg1097-106 PubMed ID 23404867
