Anti-Human IL-2 (Monoclonal Mouse) 2 ReviewsSubmit a Review Product Details Catalog Number: 500-M02 Description: Preparation: Produced in mice immunized with highly pure Recombinant Human IL-2. Anti-Human IL-2-specific antibody was purified from cell culture by Protein A affinity chromatography. Source: Monoclonal Mouse Immunogen: E.coli derived Recombinant Human IL-2 (PeproTech catalog# 200-02) Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-2 when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human IL-2 (500-P22BT) as the detection antibody at a concentration of approximately 1.0- 2.0 μg/ml. Western Blot: To detect Human IL-2 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. Cross Reactivity Cited in References: Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 AIDS/HIV Cancer Cell Culture Diabetes/Weight Regulation Immune System Inflammation Neurobiology Proliferation Stem Cells & Differentiation Wound Healing Allergy Transplantation Recent Citations First Author Tang, T Title Impaired thymic export and apoptosis contribute to regulatory T-cell defects in patients with chronic heart failure. Citation PLoS ONE; 6(9) pge24272 PubMed ID 21935395 Product Reviews Existing reviews Worked on Cultured PBMCs Had a great experience using this cytokine with cultured PBMCs. Used it at 10ng/mL and saw good viability and downstream response to stimulation with PMA/Ionomycin. From: Etienne | Date: 13/07/2020 × Worked on Cultured PBMCs Had a great experience using this cytokine with cultured PBMCs. Used it at 10ng/mL and saw good viability and downstream response to stimulation with PMA/Ionomycin. From: Etienne | Date: 13/07/2020 Close Worked on Cultured PBMCs Had a great experience using this cytokine with cultured PBMCs. Used it at 10ng/mL and saw good viability and downstream response to stimulation with PMA/Ionomycin. From: Etienne | Date: 13/07/2020 × Worked on Cultured PBMCs Had a great experience using this cytokine with cultured PBMCs. Used it at 10ng/mL and saw good viability and downstream response to stimulation with PMA/Ionomycin. From: Etienne | Date: 13/07/2020 Close
