PeproTech offers three separate endothelial cell culture media formulations developed for the in vitro cultivation of: endothelial progenitor cells (EPC; PeproGrow EPC) derived from bone marrow or peripheral blood; endothelial cells from large vessels (PeproGrow MacroV); and endothelial cells from small vessels (PeproGrow MicroV). These media formulations maintain outstanding endothelial cell morphology and function, and increase the activity of endothelial nitric oxide synthase (eNOS), which account for a specific, crucial marker for endothelial cells. By doing this, the media provide an optimal cell culture environment for macrovascular and microvascular endothelial cells, as well as for EPC; growing cells at rates that exceed commercially available media. These media formulations are optimized for primary/progenitor human cells, but can also be used for bovine, porcine, rabbit, rat, and mouse EPC, or mature endothelial cells.
PeproGrow MacroV is supplied as a 500mL bottle of basal medium and a separate, frozen growth factor component.
PeproGrow MacroV is recommended for Macrovascular Endothelial Cells:
Human Umbilical Vein Endothelial Cells (HUVEC)
Human Umbilical Artery Endothelial Cells (HUAEC)
Human Aortic Endothelial Cells (HAoEC)
Human Pulmonary Artery Endothelial Cells (HPAEC)
Human Saphenous Vein Endothelial Cells (HSaVEC)
And other large vessel (macrovascular) endothelial cells
Figure 1: HUVEC Proliferation. HUVEC were seeded onto fibronectin-coated plates and incubated for 7 days in PeproGrow MacroV basal medium + Growth Supplement MacroV and two competitors' medium. The proliferation ability of HUVEC was assessed 7 days after cell cultivation using the XTT assay following the manufacturer’s protocol. The proliferation ability of HUVEC was expressed as the average of fluorescence intensity (O.D.) calculated using a plate reader from two independent assays run in triplicates.