Registration for PeproTech.com is no longer available. To order your PeproTech products online, register or logon to ThermoFisher.com.

Growth Factors & Cytokines

Recombinant Human FGF-10

제품 상세

카다로그 번호 100-26
제품 설명
Recombinant Human FGF-10

FGF-10 is a heparin-binding growth factor that belongs to the FGF family.  Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-10 is most related to KGF/FGF-7, and is expressed during the development and, preferentially, in adult lungs. It signals through FGFR 2b. Recombinant Human FGF-10 is a 19.3 kDa protein consisting of 170 amino acid residues.

Source: E.coli

Synonyms: Fibroblast Growth Factor-10, FGFA, Keratinocyte growth factor-2

AA Sequence: MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Assay #1: Determined by dose-dependent ability to reduce tetrazolium salt, WST-8, by dehydrogenase activities of BaF3 cells expressing FGF receptors using Cell Counting Kit-8 (CCK-8). The expected ED50 is 5 ng/ml corresponding to a specific activity of ≥ 2 x 10^6 units/mg.
Assay #2:Determined by a cell proliferation assay using 4MBr-5 cells. The expected ED50 for this effect is 0.5-15.0 ng/ml.

Recombinant Human FGF-10 Biological Activity Graph

Calculated Molecular Weight: 19.3 kDa

Accession Number: O15520

Gene ID: 2255

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

제 1 저자
Fujimura, T
주제
Identifying a Lung Stem Cell Subpopulation by Combining Single-Cell Morphometrics, Organoid Culture, and Transcriptomics.
인용
Stem cells (Dayton, Ohio);
PubMed ID
제 1 저자
Tanaka, J
주제
Human induced pluripotent stem cell-derived salivary gland organoids model SARS-CoV-2 infection and replication.
인용
Nature Cell Biology; 24(11) pg1595-1605
PubMed ID
제 1 저자
Nguyen, V V
주제
A human kidney and liver organoid-based multi-organ-on-a-chip model to study the therapeutic effects and biodistribution of mesenchymal stromal cell-derived extracellular vesicles.
인용
Journal Of Extracellular Vesicles; 11(11) pge12280
PubMed ID

product.subtitle.productreviews

기존 리뷰
High quality product
Critical components of organoid media are a set of growth factors and I've used products from peprotech for 6 years to culture colon and lung organoid. I'm really satisfied with all the growth factors from peprotech and my consistent results.
에서:  Eun Joo | 날짜:  2020-07-08
organoids
All batches/lots that we have used so far worked as expected for developing pancreatic oragnoids.
에서:  Mitesh | 날짜:  2020-07-09
Excellent quality
We utilize FGF10 as part of a specialized lung cancer organoid media for cancers which do not have EGFR mutations. We have had great results and excellent service from Peprotech.
에서:  Dora | 날짜:  2020-07-24
FGF-10 for Organoid Culture
We using human FGF-10 for Pancreas, Head and Neck, Lung Organoid culture media. Works for both human and mouse medias.
Used on more than 200 organoid lines
Consistent activity and next day delivery.
Very cost effective than other suppliers.
에서:  Hardik | 날짜:  2020-08-14
hFGF-10 for pancreatic organoids culture
We have used this product as part of our organoid cell culture media and it works perfectly.
에서:  Pilar | 날짜:  2020-08-27
Organoid culture
Great for organoid culture (endometrial cells). Fast turn around on getting the product.
에서:  Juan | 날짜:  2020-08-28
Great for organoids!
We use this in most of our patient tumor organoid media and have wonderful results.
에서:  Julie-Ann | 날짜:  2021-08-03