Animal-Free Recombinant Murine LIF 0 리뷰Submit a Review 제품 상세 카다로그 번호 AF-250-02 제품 설명 LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Murine LIF is a 19.9 kDa protein containing 180 amino acids residues, including three disulfide bonds. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Leukemia Inhibitory Factor, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLPLI), Interleukin 6 family cytokine AA Sequence: SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: PeproTech's Murine LIF is fully biologically active when compared to standards. The ED50 as determined by the M1 cell differentiation assay is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg. Calculated Molecular Weight: 19.6 kDa Accession Number: P09056 Gene ID: 16878 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) crossreactivity: Mouse References PubMed SDS CoA 검색 Product Line Country Of Origin: USA Not for human use. Research Interest Diabetes/Weight Regulation Immune System Lipid Metabolism Stem Cells & Differentiation product.subtitle.recentcitations 제 1 저자 Kim, P G 주제 Signaling axis involving Hedgehog, Notch, and Scl promotes the embryonic endothelial-to-hematopoietic transition. 인용 Proceedings of the National Academy of Sciences of the United States of America; 110(2) pgE141-50 PubMed ID 23236128
