PeproGrow AF-CHO is an animal component-free, serum-free, complete medium formulation for the in vitro cultivation of Chinese Hamster Ovary-S cells (Thermo Fisher Scientific catalog numbers 11619-012, R800-07, or A11557-01). This medium is intended for recombinant protein expression in suspension culture. This complete medium contains L-alanyl-L-Glutamine, amino acids, vitamins, salts, and non-animal derived hydrolysates which is recommended for a 5 day batch culture with a seeding density of 0.5 x106 cells/mL. An adaptation process is not required for Thermo Fisher Scientific CHO-S cells.
These graphs illustrate CHO-S cell density and percent viability of C1 Inhibitor producing cells in PeproGrow CD-CHO, PeproGrow AF-CHO, and competitor's CD-CHO. Cell expression varies by protein product.