PeproGrow HEK293 is an animal component-free, serum-free, protein-free, chemically-defined, complete formulation for the in vitro cultivation of HEK293 cells (Thermo Fisher Scientific
FreeStyle TM 293-F cells, catalog number R790-07). This medium is intended for recombinant protein expression in suspension culture, which is recommended for a 5 day batch culture with a seeding density of 0.6 x106 cells/mL. This ready-to-use medium contains L-alanyl-L-Glutamine, amino acids, vitamins, and salts. An adaptation process is not required for Thermo Fisher Scientific FreeStyle TM 293-F cells.
These graphs illustrate the HEK293 BMP-6 expression in PeproGrow HEK293 medium and competitor CD-293 medium.