PeproGrow hESC is a serum- and phenol red-free medium of a complete, chemically-defined formulation designed for feeder-free maintenance and expansion of both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs) using BD Matrigel™ as a surface-coating matrix. This medium is intended for the culturing of hESCs and iPSCs in the undifferentiated, pluripotent state (SSEA4+/Oct4+), and demonstrates less than 15% spontaneous differentiation as indicated by flow cytometry. The proprietary formulation of the medium includes relevant growth factors, such as FGF2 (FGF-basic), but does not contain the insulin found in the majority of other hESC/iPSC media currently available on the market. PeproGrow hESC, which was designed and developed by PeproTech in collaboration with the Stem Cell Training Course at Rutgers University, is supplied as a 100mL, or 500mL, bottle of basal medium and a separate, lyophilized growth factor component.
These images illustrate immunostaining of iPSCs cultured in PeproGrow hESC media.