PeproGrow Animal-Free Low Protein hESC Medium is an animal component-free, serum- and phenol red-free medium of a complete, chemically-defined formulation designed for feeder-free maintenance and expansion of both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs). This medium formulation is best used with PeproTech’s Animal-Free Human Vitronectin Matrix as a surface-coating reagent; however, it can also be used with Corning Matrigel® or Synthemax® II-SC. This medium is intended for the culturing of hESCs and iPSCs in the undifferentiated, pluripotent state (Nanog+/Lin28+/Tra-1-60+ or SSEA4+/Oct4+), and demonstrates less than 15% spontaneous differentiation as indicated by immunofluorescent staining. Similar to the PeproGrow hESC Medium (Catalog Number hESC), this proprietary formulation includes relevant growth factors, such as FGF2 (FGF-basic), but does not contain the insulin found in the majority of other hESC/iPSC media currently available on the market. This medium does not contain albumin so researchers may need to adjust laboratory techniques that were developed for albumin-containing media. PeproGrow Animal-Free Low Protein hESC Medium, which was designed and developed by PeproTech in collaboration with the Stem Cell Training Course at Rutgers University, is supplied as a 100mL, or 500mL, bottle of basal medium and a separate, lyophilized growth factor component. Additional companion products, such as the Animal-Free Human Vitronectin Matrix and Buffer Kit, and the Cell Passaging/Non-Enzymatic Detachment Buffer, are available separately.
These imagess illustrate immunostaining of iPSCs cultured with PeproGrow Animal-Free Low Protein hESC media.