PeproTech offers three separate endothelial cell culture media formulations developed for the in vitro cultivation of: endothelial progenitor cells (EPC; PeproGrow EPC) derived from bone marrow or peripheral blood; endothelial cells from large vessels (PeproGrow MacroV); and endothelial cells from small vessels (PeproGrow MicroV). These media formulations maintain outstanding endothelial cell morphology and function, and increase the activity of endothelial nitric oxide synthase (eNOS), which account for a specific, crucial marker for endothelial cells. By doing this, the media provide an optimal cell culture environment for macrovascular and microvascular endothelial cells, as well as for EPC; growing cells at rates that exceed commercially available media. These media formulations are optimized for primary/progenitor human cells, but can also be used for bovine, porcine, rabbit, rat, and mouse EPC, or mature endothelial cells.
PeproGrow MicroV is supplied as a 500mL bottle of basal medium and a separate, frozen growth factor component.
PeproGrow MicroV is recommended for Microvascular Endothelial Cells:
Human Coronary Artery Endothelial Cells (HCAEC)
Human Pancreatic Microvascular Endothelial Cells (HPaMEC)
Human Dermal Microvascular Endothelial Cells (HDMEC)
Human Pulmonary Microvascular Endothelial Cells (HPMEC)
Human Dermal Lymphatic Endothelial Cells (HDLEC)
Human Brain Microvascular Endothelial Cells (HBMEC)
And other small vessel (microvascular) endothelial cells
Figures 1 and 2: HDMEC Proliferation. HDMEC were seeded onto fibronectin-coated plates and incubated for 7 days in PeproGrow MicroV basal medium + Growth Supplement-MicroV and a competitor’s medium. The proliferation ability of HDMEC was assessed 7 days after cell cultivation using the XTT assay following the manufacturer’s protocol. The proliferation ability of HDMEC was expressed as the average of fluorescence intensity (O.D.) calculated using a plate reader from two independent assays run in triplicates.