Animal-Free Recombinant Human EGF 5 ReviewsSubmit a Review Product Details Catalog Number: AF-100-15 Description: EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). Recombinant Human EGF is a 6.2 kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide bonds. *Animal-Free Recombinant Human EGF (Catalog Number AF-100-15) has replaced Recombinant Human EGF (Catalog Number 100-15) Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Epidermal Growth Factor, Urogastrone, URG AA Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 6.2 kDa Accession Number: P01133 Gene ID: 1950 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) Cross Reactivity Cited in References: Chicken, Cow, Dog, Hamster, Horse, Human, Human + Hamster, Monkey, Mouse, Pig, Rabbit, Rat, Salamander, Snake, Tunicate, Zebra Fish References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Cancer Cell Culture Immune System Inflammation Neurobiology Stem Cells & Differentiation Wound Healing Diabetes/Weight Regulation Recent Citations First Author Niu, Y Title Multiparametric and accurate functional analysis of genetic sequence variants using CRISPR-Select. Citation Nature Genetics; 54(12) pg1983-1993 PubMed ID 36471068 First Author Hanson, R L Title Coordination of MAPK and p53 dynamics in the cellular responses to DNA damage and oxidative stress. Citation Molecular Systems Biology; 18(12) pge11401 PubMed ID 36472304 First Author Zhang, Z Title Ti3 C2 Tx MXene Composite 3D Hydrogel Potentiates mTOR Signaling to Promote the Generation of Functional Hair Cells in Cochlea Organoids. Citation Advanced science (Weinheim, Baden-Wurttemberg, Germany); 9(32) pge2203557 PubMed ID 36117048 Product Reviews Existing reviews Quality EGF for Organoid Culture one of best quality growth factors from peprotech, we are using for various organoid media. Consistent quality, very very cost effective than competitor suppliers, next day delivery. From: Hardik | Date: 8/14/2020 × Quality EGF for Organoid Culture one of best quality growth factors from peprotech, we are using for various organoid media. Consistent quality, very very cost effective than competitor suppliers, next day delivery. From: Hardik | Date: 8/14/2020 Close Constant quality for in vitro culture Using for in vitro culture. Constant quality. Also quick delivery. From: KOHSUKE | Date: 8/24/2021 × Constant quality for in vitro culture Using for in vitro culture. Constant quality. Also quick delivery. From: KOHSUKE | Date: 8/24/2021 Close EGF for primary cell culture I have used Peprotech human recombinant EGF and FGF for the culture of primary glioblastoma cells for several years now. Works well, no complaints and any questions I have had have been answered quickly and thoroughly by Peprotech customer service From: Kathleen | Date: 8/24/2021 × EGF for primary cell culture I have used Peprotech human recombinant EGF and FGF for the culture of primary glioblastoma cells for several years now. Works well, no complaints and any questions I have had have been answered quickly and thoroughly by Peprotech customer service From: Kathleen | Date: 8/24/2021 Close EGF, human basic recombinant, E. coli Using the EGF for 5 years now in our culture medium and are very satisfied ! Critical cells, but they grow very well with the EGF (and FGF) of Peprotech ! From: Arjan | Date: 8/27/2021 × EGF, human basic recombinant, E. coli Using the EGF for 5 years now in our culture medium and are very satisfied ! Critical cells, but they grow very well with the EGF (and FGF) of Peprotech ! From: Arjan | Date: 8/27/2021 Close Great recombinant protein Works very nicely in combination with FGF to keep mouse ESC-derived NPCs alive and proliferating in culture (at 10 ng/ml concentration). Highly recommend. From: Epp | Date: 8/31/2021 × Great recombinant protein Works very nicely in combination with FGF to keep mouse ESC-derived NPCs alive and proliferating in culture (at 10 ng/ml concentration). Highly recommend. From: Epp | Date: 8/31/2021 Close
