Animal-Free Cytokines

Animal-Free Recombinant Human FGF-basic (154 a.a.)

Product Details

Catalog Number: AF-100-18B
Description:

FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated, heparin-binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant Human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues.

Qualified for stem cell use.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin

AA Sequence: AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Purity: Greater than 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 0.5ng/ml corresponding to a specific activity of ≥ 2 x 106 units/mg.

Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

Cross Reactivity:
Chicken, Cow, Dog, Frog, Hamster, Horse, Human, Human + Mouse, Human + Virus, Monkey, Mouse, N/A, Pig, Rabbit, Rat, Rat + Chicken, Salamander, Sheep
Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.