LAG-1 is CC chemokine that signals through the CCR5 receptor. LAG-1 is identical to MIP-1β (ACT II isotype) except for two amino acid substitutions; arginine for histidine at position 22 and serine for glycine at position 47 of the mature protein. LAG-1 chemoattracts monocytes, and exhibits activity as an HIV-suppressive factor. Recombinant Human LAG-1 is a 7.7 kDa protein containing 69 amino acid residues.
Lymphocyte Activation Gene-1, CCL4L1, ACT-2, T-cell activiation-2, HC21
APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN
≥ 98% by SDS-PAGE gel and HPLC analyses.
Determined by its ability to chemoattract CCR5-transfected cells using a concentration of 0.1-10.0 ng/ml