Recombinant Human MMP-3 0 ReviewsSubmit a Review Product Details Catalog Number: 420-03 Description: Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-3 degrades fibronectin, laminin, collagens III, IV, and X, and cartilage proteoglycans. Recombinant Human MMP-3 is a 42.8 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain (378 amino acids). Source: E.coli Synonyms: Matrix Metalloproteinase-3, Stromelysin-1, SL-1, Transin-1 AA Sequence: MRTFPGIPKW RKTHLTYRIV NYTPDLPKDA VDSAVEKALK VWEEVTPLTF SRLYEGEADI MISFAVREHG DFYPFDGPGN VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE IGHSLGLFHS ANTEALMYPL YHSLTDLTRF RLSQDDINGI QSLYGPPPDS PETPLVPTEP VPPEPGTPAN CDPALSFDAV STLRGEILIF KDRHFWRKSL RKLEPELHLI SSFWPSLPSG VDAAYEVTSK DLVFIFKGNQ FWAIRGNEVR AGYPRGIHTL GFPPTVRKID AAISDKEKNK TYFFVEDKYW RFDEKRNSME PGFPKQIAED FPGIDSKIDA VFEEFGFFYF FTGSSQLEFD PNAKKVTHTL KSNSWLNC Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: MMP-3 activity was measured by its ability to cleave a chromogenic peptide MMP-3 substrate at room temperature. At an MMP-3 concentration of 2.5 μg/ml, 50% cleavage was achieved at an incubation time of approximately 75 minutes. Calculated Molecular Weight: 42.8 kDa Accession Number: P08254 Gene ID: 4314 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Cross Reactivity Cited in References: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Bone, Skeletal, Cartilage Cancer Immune System Inflammation Wound Healing Recent Citations First Author Shang, D Title Activation of epidermal growth factor receptor signaling mediates cellular senescence induced by certain pro-inflammatory cytokines. Citation Aging Cell; 19(5) pge13145 PubMed ID 32323422 First Author Przybyla, L M Title Matrix remodeling maintains embryonic stem cell self-renewal by activating Stat3. Citation Stem cells (Dayton, Ohio); 31(6) pg1097-106 PubMed ID 23404867
