Animal-Free Cytokines

Animal-Free Recombinant Human β-NGF

Product Details

Catalog Number: AF-450-01
Description:

β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4.  These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures.  β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.  β-NGF also acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival.  The functional form of Recombinant Human β-NGF is a non-covalently-linked homodimer of two 13.5 kDa, polypeptide monomers that each contain 120 amino acids and three disulfide bonds, which are required for biological activity.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: NGF-β, beta-Nerve Growth Factor

AA Sequence (monomer): SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVR

Purity: Greater than 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

Cross Reactivity:
Chicken, Hamster, Human, Mouse, Rat
Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.