SCGF-α and -β are hematopoietic growth factors that exert their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated, species-specific cytokines that can support growth of primitive hematopoietic cells, and, in combination with EPO or GM-CSF, promote proliferation of erythroid or myeloid progenitors, respectively. Recombinant Human SCGF-α is a 33.9 kDa polypeptide containing 305 amino acid residues.
Stem Cell Growth Factor-α
GHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ AAAQARCTAR GGSLAQPADR QQMEALTRYL RAALAPYNWP VWLGVHDRRA EGLYLFENGQ RVSFFAWHRS PRPELGAQPS ASPHPLSPDQ PNGGTLENCV AQASDDGSWW DHDCQRRLYY VCEFPF
≥ 98% by SDS-PAGE gel and HPLC analyses.
Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed at ≤ 2.5 ng/ml.