Recombinant Human sRANK Ligand (CHO derived) 0 ReviewsSubmit a Review Product Details Catalog Number: 310-01C Description: RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic cell-membrane protein, which, by interacting with RANKL, augments the ability of dendritic cells. These dendritic cells then stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, promote the survival of RANK+ T-cells, and regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells,including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Human RANKL is reactive on murine cells. PeproTech's CHO cell-derived Recombinant Human sRANK Ligand is a 22.1 kDa polypeptide comprised of the TNF-homologous region of RANKL and a N-terminal His-tag. Source: CHO cells Synonyms: soluble Receptor Activator of NF-κB Ligand, TNFSF11, TRANCE (TNF-Related Activation-induced Cytokine), OPGL, ODF (Osteoclast Differentiation Factor), CD254 AA Sequence: HHHHHHHHPS PGGSGGSQHI RAEKAMVDGS WLDLAKRSKL EAQPFAHLTI NATDIPSGSH KVSLSSWYHD RGWGKISNMT FSNGKLIVNQ DGFYYLYANI CFRHHETSGD LATEYLQLMV YVTKTSIKIP SSHTLMKGGS TKYWSGNSEF HFYSINVGGF FKLRSGEEIS IEVSNPSLLD PDQDATYFGA FKVRDID Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its dose-dependent ability to induce reporter gene in HT-29 NF-κB Luc reporter cells. Calculated Molecular Weight: 22.1 kDa Accession Number: O14788 Gene ID: 8600 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Cross Reactivity Cited in References: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Apoptosis Bone, Skeletal, Cartilage Cancer Diabetes/Weight Regulation Immune System TNF Superfamily Recent Citations First Author Kaur, K Title Osteoclast-expanded super-charged NK-cells preferentially select and expand CD8+ T cells. Citation Scientific Reports; 10(1) pg20363 PubMed ID 33230147 First Author Yang, B Title Amyloid β peptide promotes bone formation by regulating Wnt/β-catenin signaling and the OPG/RANKL/RANK system. Citation ACR Open Rheumatology; 34(3) pg3583-3593 PubMed ID 31944393 First Author Weivoda, M Title Identification of osteoclast-osteoblast coupling factors in humans reveals links between bone and energy metabolism. Citation Nature Communications; 11(1) pg87 PubMed ID 31911667