Recombinant Human TPO 1 ReviewsSubmit a Review Product Details Catalog Number: 300-18 Description: TPO is a lineage-specific growth factor produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor, and acts as an important regulator of circulating platelets. Human and murine TPO exhibit cross-species reactivity. The human TPO gene encodes for a 353 amino acid glycoprotein, which contains a 21 amino acid signal sequence, a 15 amino acid erythropoietin-like domain, and a highly glycosylated 179 amino acid C-terminal domain. Recombinant Human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein. Source: E.coli Synonyms: Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF AA Sequence: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg. Calculated Molecular Weight: 18.6 kDa Accession Number: P40225 Gene ID: 7066 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Cross Reactivity Cited in References: Human, Human + Hamster, Human + Monkey, Human + Mouse, Monkey, Mouse, Mouse + Virus, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cell Culture Immune System Stem Cells & Differentiation Wound Healing Recent Citations First Author Li, Y Title Modulation of WNT, Activin/Nodal, and MAPK Signaling Pathways Increases Arterial Hemogenic Endothelium and Hematopoietic Stem/Progenitor Cell Formation During Human iPSC Differentiation. Citation Stem cells (Dayton, Ohio); 41(7) pg685-697 PubMed ID 37220178 First Author Thomas, G E Title The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness. Citation Nature Cell Biology; 24(6) pg872-884 PubMed ID 35668135 First Author Tvorogov, D Title Targeting human CALR-mutated MPN progenitors with a neoepitope-directed monoclonal antibody. Citation EMBO reports; 23(4) pge52904 PubMed ID 35156745 Product Reviews Existing reviews TPO dans la culture Cellules souches pluripotentes J'ai utilise de la TPOhu pour la différenciation des cellules souches pluripotentes humaines vers l'hemangioblaste et les cellules hématopoïètiques.Je suis très satisfaite de ce produit (et depuis plusieures années) From: Maite | Date: 7/29/2020 × TPO dans la culture Cellules souches pluripotentes J'ai utilise de la TPOhu pour la différenciation des cellules souches pluripotentes humaines vers l'hemangioblaste et les cellules hématopoïètiques.Je suis très satisfaite de ce produit (et depuis plusieures années) From: Maite | Date: 7/29/2020 Close
