Animal-Free Recombinant Human TPO 0 ReviewsSubmit a Review Product Details Catalog Number: AF-300-18 Description: TPO is a lineage-specific growth factor produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor, and acts as an important regulator of circulating platelets. Human and murine TPO exhibit cross-species reactivity. The human TPO gene encodes for a 353 amino acid glycoprotein, which contains a 21 amino acid signal sequence, a 15 amino acid erythropoietin-like domain, and a highly glycosylated 179 amino acid C-terminal domain. Recombinant Human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF AA Sequence: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg. Calculated Molecular Weight: 18.6 kDa Accession Number: P40225 Gene ID: 7066 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) Cross Reactivity Cited in References: Human, Human + Hamster, Monkey, Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cell Culture Immune System Stem Cells & Differentiation Wound Healing Recent Citations First Author Eernstman, J Title Novel variants in Krueppel like factor 1 that cause persistence of fetal hemoglobin in In(Lu) individuals Citation Scientific Reports; 11(1) pg18557 PubMed ID 34535703 First Author McGrath, E Title Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing. Citation Nature Communications; 10(1) pg5353 PubMed ID 31767844 First Author Minuesa, G Title Small-molecule targeting of MUSASHI RNA-binding activity in acute myeloid leukemia. Citation Nature Communications; 10(1) pg2691 PubMed ID 31217428
