Twisted Gastrulation Protein (TSG) is a secreted BMP-binding protein that is structurally related to the BMP antagonists Chordin and Noggin. TSG can inhibit BMP activity by binding directly to BMP proteins, and can act either as a BMP-4 agonist or antagonist (depending on the specific biochemical environment) by binding to the BMP-4/Chordin complex. Recombinant Human TSG is a 198 amino acid, 22.2 kDa protein containing the BMP/TGF-β binding portion of the full length TSG protein.
Twisted Gastrulation Protein
CNKALCASDV SKCLIQELCQ CRPGEGNCSC CKECMLCLGA LWDECCDCVG MCNPRNYSDT PPTSKSTVEE LHEPIPSLFR ALTEGDTQLN WNIVSFPVAE ELSHHENLVS FLETVNQPHH QNVSVPSNNV HAPYSSDKEH MCTVVYFDDC MSIHQCKISC ESMGASKYRW FHNACCECIG PECIDYGSKT VKCMNCMF
≥ 98% by SDS-PAGE gel and HPLC analyses.
Determined by its ability to neutralize BMP-6-induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is ≤ 2.0 μg/ml of TSG.