Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G1 and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis. Recombinant Mycoplasma Arginine Deiminase is a 46.3 kDa protein consisting of 409 amino acids.
SVFDSKFKGI HVYSEIGELE SVLVHEPGRE IDYITPARLD ELLFSAILES HDARKEHKQF VAELKANDIN VVELIDLVAE TYDLASQEAK DKLIEEFLED SEPVLSEEHK VVVRNFLKAK KTSRELVEIM MAGITKTDLG IEADHELIVD PMPNLYFTRD PFASVGNGVT IHYMRYKVRQ RETLFSRFVF SNHPKLINTP WYYDPSLKLS IEGGDVFIYN NDTLVVGVSE RTDLQTVTLL AKNIVANKEC EFKRIVAINV PKWTNLMHLD TWLTMLDKDK FLYSPIANDV FKFWDYDLVN GGAEPQPVEN GLPLEGLLQS IINKKPVLIP IAGEGASQME IERETHFDGT NYLAIRPGVV IGYSRNEKTN AALEAAGIKV LPFHGNQLSL GMGNARCMSM PLSRKDVKW
≥ 97% by SDS-PAGE gel and HPLC analyses.
Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml.