Full length and fully biologically active RUO cytokines are developed in-house by our experienced protein scientists. The process starts in our molecular biology labs with gene design and expression, to cell banking, and continues to fermentation/cell culture, purification and finally QC testing.
E.coli expressed proteins:
With over 15,000 sq. ft. of fermentation, protein processing and purification facilities, PeproTech is able to meet the demands of its clients.
Cell culture expressed proteins:
With state-of-the-art cell culture and protein purification facilities, PeproTech is also able to provide glycosylated fully biological active proteins.
PeproTech’s Animal-Free product line is designed to minimize potential risks that can arise from trace amounts of animal-derived adventitious agents, such as viruses, TSE/BSE and unknowns.
Expressed in E.coli bacterial culture, Animal-Free recombinant proteins are manufactured in a dedicated Animal-Free facility under strict Animal-Free conditions where the production protocols have been modified to include only Animal-Free reagents and chemicals. All product contact material is also qualified as animal component free.
PeproTech’s Animal-Free proteins maintain identical high biological activity and purity to the corresponding proteins that are produced using standard techniques that equal or enhance the performance of animal-derived media formulations. Products are lyophilized, with a minimum amount of salt, and without additives or preservatives added.
PeproTech’s RUO cytokines are subject to a rigorous set of quality control standards. Our Quality Assurance department ensures that the cytokines demonstrate consistent molecular weights, N-terminal sequences, purity and biological activity. This quality control is devoted to providing consistency between lots and the following qualities:
RUO cytokine products are verified by N-terminal sequence analysis, SDS-PAGE and, where possible, by RP-HPLC, Mass Spectrometry, and Western Blot analyses versus standards.
Determined in the relevant in vitro and in vivo bioassay.
Tested by kinetic LAL (Limulus Amebocyte Lysate) method to ensure the lowest possible endotoxin levels and the highest purity.
Verified by UV spectroscopy, SDS-PAGE and, where possible, by RP-HPLC.
Verified by SDS-PAGE and, where possible, by RP-HPLC.
All products are sterile filtered through a 0.2 micron filter.